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skeletal muscle cell basal medium  (PromoCell)


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    PromoCell skeletal muscle cell basal medium
    a Immunostaining result of CHAMP1 (top row), MyoG (middle row) and Myosin (MF20, gray signal in bottom row) for human myoblasts in growth <t>medium</t> or at various time points post myogenic induction. Scale bar: 100 μm. b Schematic of the human CHAMP1 protein and its domains, with the predicted CRISPR targeting sites for three selected gRNAs indicated by red arrows. The antibody recognition site at the C-terminus of the protein was highlighted. C2H2-ZNF: Cys2–His2 zinc-finger motif; WK, SPE, and FPE motifs are named after their consensus residues. c Representative immunostaining result. Numbers in the top panels denote CRISPR-induced indel sizes (bp) for the two CHAMP1 alleles (e.g., −14/ − 5 indicates 14-bp and 5-bp deletions on the two alleles). Scale bar, 10 μm for the top row and 100 μm for middle and bottom rows. Arrows point to multinucleated myotubes. GM: growth medium; DM: <t>muscle</t> differentiation medium. Quantification of myoblast differentiation ( d ) and fusion ( e ) at day 6 post myogenic differentiation from three independent experiments. The fusion index is normalized to the number of nuclei expressing myosin. WT: wildtype. f Design of human myoblast transplantation experiment. The hindlimb of the immunodeficient mice were pre-irradiated and pre-injured to eliminate endogenous muscle stem cells and promote human myoblast engraftment. g Representative immunostaining results of human myoblast transplantation experiments. LAMIN A/C labels human nucleus after transplantation; MYH3 marks regenerating muscle cells; laminin delineates the muscle <t>cell</t> periphery. Scale bar: 10 μm. h , Distribution of cross-sectional area of human myofibers from transplantation experiments. n = 4 for each genotype. P values were calculated using one-way ANOVA followed by Tukey’s multiple comparisons test ( d , e ) and two-sided unpaired Student’s t test ( h ). Data are presented as mean ± s.d. Source data are provided as a Source Data file.
    Skeletal Muscle Cell Basal Medium, supplied by PromoCell, used in various techniques. Bioz Stars score: 94/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/skeletal muscle cell basal medium/product/PromoCell
    Average 94 stars, based on 54 article reviews
    skeletal muscle cell basal medium - by Bioz Stars, 2026-02
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    1) Product Images from "CHAMP1 is an essential regulator for human myoblast fusion and muscle development"

    Article Title: CHAMP1 is an essential regulator for human myoblast fusion and muscle development

    Journal: Nature Communications

    doi: 10.1038/s41467-025-67584-w

    a Immunostaining result of CHAMP1 (top row), MyoG (middle row) and Myosin (MF20, gray signal in bottom row) for human myoblasts in growth medium or at various time points post myogenic induction. Scale bar: 100 μm. b Schematic of the human CHAMP1 protein and its domains, with the predicted CRISPR targeting sites for three selected gRNAs indicated by red arrows. The antibody recognition site at the C-terminus of the protein was highlighted. C2H2-ZNF: Cys2–His2 zinc-finger motif; WK, SPE, and FPE motifs are named after their consensus residues. c Representative immunostaining result. Numbers in the top panels denote CRISPR-induced indel sizes (bp) for the two CHAMP1 alleles (e.g., −14/ − 5 indicates 14-bp and 5-bp deletions on the two alleles). Scale bar, 10 μm for the top row and 100 μm for middle and bottom rows. Arrows point to multinucleated myotubes. GM: growth medium; DM: muscle differentiation medium. Quantification of myoblast differentiation ( d ) and fusion ( e ) at day 6 post myogenic differentiation from three independent experiments. The fusion index is normalized to the number of nuclei expressing myosin. WT: wildtype. f Design of human myoblast transplantation experiment. The hindlimb of the immunodeficient mice were pre-irradiated and pre-injured to eliminate endogenous muscle stem cells and promote human myoblast engraftment. g Representative immunostaining results of human myoblast transplantation experiments. LAMIN A/C labels human nucleus after transplantation; MYH3 marks regenerating muscle cells; laminin delineates the muscle cell periphery. Scale bar: 10 μm. h , Distribution of cross-sectional area of human myofibers from transplantation experiments. n = 4 for each genotype. P values were calculated using one-way ANOVA followed by Tukey’s multiple comparisons test ( d , e ) and two-sided unpaired Student’s t test ( h ). Data are presented as mean ± s.d. Source data are provided as a Source Data file.
    Figure Legend Snippet: a Immunostaining result of CHAMP1 (top row), MyoG (middle row) and Myosin (MF20, gray signal in bottom row) for human myoblasts in growth medium or at various time points post myogenic induction. Scale bar: 100 μm. b Schematic of the human CHAMP1 protein and its domains, with the predicted CRISPR targeting sites for three selected gRNAs indicated by red arrows. The antibody recognition site at the C-terminus of the protein was highlighted. C2H2-ZNF: Cys2–His2 zinc-finger motif; WK, SPE, and FPE motifs are named after their consensus residues. c Representative immunostaining result. Numbers in the top panels denote CRISPR-induced indel sizes (bp) for the two CHAMP1 alleles (e.g., −14/ − 5 indicates 14-bp and 5-bp deletions on the two alleles). Scale bar, 10 μm for the top row and 100 μm for middle and bottom rows. Arrows point to multinucleated myotubes. GM: growth medium; DM: muscle differentiation medium. Quantification of myoblast differentiation ( d ) and fusion ( e ) at day 6 post myogenic differentiation from three independent experiments. The fusion index is normalized to the number of nuclei expressing myosin. WT: wildtype. f Design of human myoblast transplantation experiment. The hindlimb of the immunodeficient mice were pre-irradiated and pre-injured to eliminate endogenous muscle stem cells and promote human myoblast engraftment. g Representative immunostaining results of human myoblast transplantation experiments. LAMIN A/C labels human nucleus after transplantation; MYH3 marks regenerating muscle cells; laminin delineates the muscle cell periphery. Scale bar: 10 μm. h , Distribution of cross-sectional area of human myofibers from transplantation experiments. n = 4 for each genotype. P values were calculated using one-way ANOVA followed by Tukey’s multiple comparisons test ( d , e ) and two-sided unpaired Student’s t test ( h ). Data are presented as mean ± s.d. Source data are provided as a Source Data file.

    Techniques Used: Immunostaining, CRISPR, Cell Characterization, Expressing, Transplantation Assay, Irradiation



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    a Immunostaining result of CHAMP1 (top row), MyoG (middle row) and Myosin (MF20, gray signal in bottom row) for human myoblasts in growth <t>medium</t> or at various time points post myogenic induction. Scale bar: 100 μm. b Schematic of the human CHAMP1 protein and its domains, with the predicted CRISPR targeting sites for three selected gRNAs indicated by red arrows. The antibody recognition site at the C-terminus of the protein was highlighted. C2H2-ZNF: Cys2–His2 zinc-finger motif; WK, SPE, and FPE motifs are named after their consensus residues. c Representative immunostaining result. Numbers in the top panels denote CRISPR-induced indel sizes (bp) for the two CHAMP1 alleles (e.g., −14/ − 5 indicates 14-bp and 5-bp deletions on the two alleles). Scale bar, 10 μm for the top row and 100 μm for middle and bottom rows. Arrows point to multinucleated myotubes. GM: growth medium; DM: <t>muscle</t> differentiation medium. Quantification of myoblast differentiation ( d ) and fusion ( e ) at day 6 post myogenic differentiation from three independent experiments. The fusion index is normalized to the number of nuclei expressing myosin. WT: wildtype. f Design of human myoblast transplantation experiment. The hindlimb of the immunodeficient mice were pre-irradiated and pre-injured to eliminate endogenous muscle stem cells and promote human myoblast engraftment. g Representative immunostaining results of human myoblast transplantation experiments. LAMIN A/C labels human nucleus after transplantation; MYH3 marks regenerating muscle cells; laminin delineates the muscle <t>cell</t> periphery. Scale bar: 10 μm. h , Distribution of cross-sectional area of human myofibers from transplantation experiments. n = 4 for each genotype. P values were calculated using one-way ANOVA followed by Tukey’s multiple comparisons test ( d , e ) and two-sided unpaired Student’s t test ( h ). Data are presented as mean ± s.d. Source data are provided as a Source Data file.
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    a Immunostaining result of CHAMP1 (top row), MyoG (middle row) and Myosin (MF20, gray signal in bottom row) for human myoblasts in growth <t>medium</t> or at various time points post myogenic induction. Scale bar: 100 μm. b Schematic of the human CHAMP1 protein and its domains, with the predicted CRISPR targeting sites for three selected gRNAs indicated by red arrows. The antibody recognition site at the C-terminus of the protein was highlighted. C2H2-ZNF: Cys2–His2 zinc-finger motif; WK, SPE, and FPE motifs are named after their consensus residues. c Representative immunostaining result. Numbers in the top panels denote CRISPR-induced indel sizes (bp) for the two CHAMP1 alleles (e.g., −14/ − 5 indicates 14-bp and 5-bp deletions on the two alleles). Scale bar, 10 μm for the top row and 100 μm for middle and bottom rows. Arrows point to multinucleated myotubes. GM: growth medium; DM: <t>muscle</t> differentiation medium. Quantification of myoblast differentiation ( d ) and fusion ( e ) at day 6 post myogenic differentiation from three independent experiments. The fusion index is normalized to the number of nuclei expressing myosin. WT: wildtype. f Design of human myoblast transplantation experiment. The hindlimb of the immunodeficient mice were pre-irradiated and pre-injured to eliminate endogenous muscle stem cells and promote human myoblast engraftment. g Representative immunostaining results of human myoblast transplantation experiments. LAMIN A/C labels human nucleus after transplantation; MYH3 marks regenerating muscle cells; laminin delineates the muscle <t>cell</t> periphery. Scale bar: 10 μm. h , Distribution of cross-sectional area of human myofibers from transplantation experiments. n = 4 for each genotype. P values were calculated using one-way ANOVA followed by Tukey’s multiple comparisons test ( d , e ) and two-sided unpaired Student’s t test ( h ). Data are presented as mean ± s.d. Source data are provided as a Source Data file.
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    Image Search Results


    a Immunostaining result of CHAMP1 (top row), MyoG (middle row) and Myosin (MF20, gray signal in bottom row) for human myoblasts in growth medium or at various time points post myogenic induction. Scale bar: 100 μm. b Schematic of the human CHAMP1 protein and its domains, with the predicted CRISPR targeting sites for three selected gRNAs indicated by red arrows. The antibody recognition site at the C-terminus of the protein was highlighted. C2H2-ZNF: Cys2–His2 zinc-finger motif; WK, SPE, and FPE motifs are named after their consensus residues. c Representative immunostaining result. Numbers in the top panels denote CRISPR-induced indel sizes (bp) for the two CHAMP1 alleles (e.g., −14/ − 5 indicates 14-bp and 5-bp deletions on the two alleles). Scale bar, 10 μm for the top row and 100 μm for middle and bottom rows. Arrows point to multinucleated myotubes. GM: growth medium; DM: muscle differentiation medium. Quantification of myoblast differentiation ( d ) and fusion ( e ) at day 6 post myogenic differentiation from three independent experiments. The fusion index is normalized to the number of nuclei expressing myosin. WT: wildtype. f Design of human myoblast transplantation experiment. The hindlimb of the immunodeficient mice were pre-irradiated and pre-injured to eliminate endogenous muscle stem cells and promote human myoblast engraftment. g Representative immunostaining results of human myoblast transplantation experiments. LAMIN A/C labels human nucleus after transplantation; MYH3 marks regenerating muscle cells; laminin delineates the muscle cell periphery. Scale bar: 10 μm. h , Distribution of cross-sectional area of human myofibers from transplantation experiments. n = 4 for each genotype. P values were calculated using one-way ANOVA followed by Tukey’s multiple comparisons test ( d , e ) and two-sided unpaired Student’s t test ( h ). Data are presented as mean ± s.d. Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: CHAMP1 is an essential regulator for human myoblast fusion and muscle development

    doi: 10.1038/s41467-025-67584-w

    Figure Lengend Snippet: a Immunostaining result of CHAMP1 (top row), MyoG (middle row) and Myosin (MF20, gray signal in bottom row) for human myoblasts in growth medium or at various time points post myogenic induction. Scale bar: 100 μm. b Schematic of the human CHAMP1 protein and its domains, with the predicted CRISPR targeting sites for three selected gRNAs indicated by red arrows. The antibody recognition site at the C-terminus of the protein was highlighted. C2H2-ZNF: Cys2–His2 zinc-finger motif; WK, SPE, and FPE motifs are named after their consensus residues. c Representative immunostaining result. Numbers in the top panels denote CRISPR-induced indel sizes (bp) for the two CHAMP1 alleles (e.g., −14/ − 5 indicates 14-bp and 5-bp deletions on the two alleles). Scale bar, 10 μm for the top row and 100 μm for middle and bottom rows. Arrows point to multinucleated myotubes. GM: growth medium; DM: muscle differentiation medium. Quantification of myoblast differentiation ( d ) and fusion ( e ) at day 6 post myogenic differentiation from three independent experiments. The fusion index is normalized to the number of nuclei expressing myosin. WT: wildtype. f Design of human myoblast transplantation experiment. The hindlimb of the immunodeficient mice were pre-irradiated and pre-injured to eliminate endogenous muscle stem cells and promote human myoblast engraftment. g Representative immunostaining results of human myoblast transplantation experiments. LAMIN A/C labels human nucleus after transplantation; MYH3 marks regenerating muscle cells; laminin delineates the muscle cell periphery. Scale bar: 10 μm. h , Distribution of cross-sectional area of human myofibers from transplantation experiments. n = 4 for each genotype. P values were calculated using one-way ANOVA followed by Tukey’s multiple comparisons test ( d , e ) and two-sided unpaired Student’s t test ( h ). Data are presented as mean ± s.d. Source data are provided as a Source Data file.

    Article Snippet: Immortalized human myoblasts are maintained in skeletal muscle cell basal medium (PromoCell, C-23260) supplemented with 15% fetal bovine serum (FBS; Sigma-Aldrich, F2442), 5% growth medium supplement mix (PromoCell, C-39365), GlutaMAX, and 1% gentamicin sulfate.

    Techniques: Immunostaining, CRISPR, Cell Characterization, Expressing, Transplantation Assay, Irradiation